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ENTPD5 Polyclonal antibody

ENTPD5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26746-1-AP

Print datasheet

Synonyms

CD39 antigen like 4, CD39L4, ENTPD5, ER UDPase, NTPDase 5, Nucleoside diphosphatase, PCPH

验证数据展示

  • WB analysis of mouse liver using 26746-1-AP

    WB analysis of mouse liver using 26746-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26746-1-AP (ENTPD5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 26746-1-AP

    IP experiment of mouse liver using 26746-1-AP

    IP result of anti-ENTPD5 (IP:26746-1-AP, 4ug; Detection:26746-1-AP 1:300) with mouse liver tissue lysate 6000ug.

  • IHC staining of human liver using 26746-1-AP

    IHC staining of human liver using 26746-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26746-1-AP (ENTPD5 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 26746-1-AP

    IHC staining of human liver using 26746-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26746-1-AP (ENTPD5 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26746-1-AP is ENTPD5 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ENTPD5 fusion protein Ag25022 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ectonucleoside triphosphate diphosphohydrolase 5
Synonyms CD39 antigen like 4, CD39L4, ENTPD5, ER UDPase, NTPDase 5, Nucleoside diphosphatase, PCPH
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC130485
Gene Symbol ENTPD5
Gene ID (NCBI) 957
RRIDAB_2880619
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75356
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ENTPD5 antibody 26746-1-APDownload protocol
IHC protocol for ENTPD5 antibody 26746-1-APDownload protocol
IP protocol for ENTPD5 antibody 26746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols