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ENTPD7 Polyclonal antibody

ENTPD7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24558-1-AP

Print datasheet

Synonyms

Lysosomal apyrase-like protein 1, LALP1, EC:3.6.1.15

验证数据展示

  • WB analysis of HeLa using 24558-1-AP

    WB analysis of HeLa using 24558-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24558-1-AP (ENTPD7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 24558-1-AP

    IHC staining of human ovary tumor using 24558-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24558-1-AP (ENTPD7 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 24558-1-AP

    IHC staining of human ovary tumor using 24558-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24558-1-AP (ENTPD7 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24558-1-AP

    IF Staining of HeLa using 24558-1-AP

    Immunofluorescent analysis of HeLa cells using 24558-1-AP (ENTPD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24558-1-AP targets ENTPD7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ENTPD7 fusion protein Ag18417 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ectonucleoside triphosphate diphosphohydrolase 7
Synonyms Lysosomal apyrase-like protein 1, LALP1, EC:3.6.1.15
Calculated Molecular Weight 604 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC122857
Gene Symbol ENTPD7
Gene ID (NCBI) 57089
RRIDAB_2879607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQZ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ectonucleoside triphosphate diphosphohydrolase 7 (ENTPD7), also known as LALP1, is a member of the ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) family. It is located in the nucleoplasm and has an effect on oxidative stress, DNA damage and ageing (PMID: 27737960).

实验方案

Product Specific Protocols
WB protocol for ENTPD7 antibody 24558-1-APDownload protocol
IHC protocol for ENTPD7 antibody 24558-1-APDownload protocol
IF protocol for ENTPD7 antibody 24558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols