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  • Featured Product
  • KD/KO Validated

ENY2 Polyclonal antibody

ENY2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15778-1-AP

Print datasheet

Synonyms

DC6, e(y)2, ENY2

验证数据展示

  • IHC staining of human breast cancer using 15778-1-AP

    IHC staining of human breast cancer using 15778-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15778-1-AP (ENY2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of PC-3 using 15778-1-AP

    WB analysis of PC-3 using 15778-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15778-1-AP (ENY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15778-1-AP targets ENY2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human
Cited Reactivityhuman
Immunogen ENY2 fusion protein Ag8462 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enhancer of yellow 2 homolog (Drosophila)
Synonyms DC6, e(y)2, ENY2
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC007870
Gene Symbol ENY2
Gene ID (NCBI) 56943
RRIDAB_2878184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPA8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENY2, also named as DC6, is involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. It is required for nuclear receptor-mediated transactivation. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). The MW of ENY2 is about 10 kDa.

实验方案

Product Specific Protocols
WB protocol for ENY2 antibody 15778-1-APDownload protocol
IHC protocol for ENY2 antibody 15778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IP

J Virol

The cellular protein complex associated with a transforming region of E1A contains c-MYC.

Authors - S Vijayalingam
  • KD Validated
View Article