EOMES/TBR2 Recombinant antibody

EOMES/TBR2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83945-5-RR

产品说明书

CloneNo. 241036D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

EOMES, TBR2, 241036D9, Eomesodermin homolog, T box brain protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inNK-92 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83945-5-RR targets EOMES/TBR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 EOMES/TBR2 fusion protein Ag27634 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eomesodermin homolog (Xenopus laevis)
别名 EOMES, TBR2, 241036D9, Eomesodermin homolog, T box brain protein 2
计算分子量 73 aa
观测分子量 67 kDa
GenBank蛋白编号NM_001278182
基因名称 EOMES
Gene ID (NCBI) 8320
RRIDAB_3671525
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO95936
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EOMES/TRB2 (also named Eomesodermin/Tbr2) encode T-box transcription factor expressed highly in the intermediate progenitor stage of the developing neuron. During migration of the neurons, radial glia divide and migrate towards the surface of the cerebral cortex, there are three stages of cellular development: radial glia, intermediate progenitors, and postmitotic projection neurons. Radial glia express Pax6, while intermediate progenitor cells express Eomesodermin/Tbr2, and postmitotic projection neurons express Tbr1 (PMID:15634788; PMID:16320258). This process also called neurogenesis, and demonstrated that Eomesodermin/Tbr2 has been implicated in neurodevelopment. According to the mouse model, several articles showed that EOMES/TRB2 KO mice having proliferating cells decreased and having smaller upper cortical layers and a smaller sub ventricular zone in the brain (PMID:18940588). What's more, Eomesodermin/Tbr2 has also been found participating in immune response (PMID:20713880).

实验方案

Product Specific Protocols
WB protocol for EOMES/TBR2 antibody 83945-5-RRDownload protocol
IHC protocol for EOMES/TBR2 antibody 83945-5-RRDownload protocol
IF protocol for EOMES/TBR2 antibody 83945-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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