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EOMES/TBR2 Recombinant antibody

EOMES/TBR2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241036D9

Cat no : 83945-5-RR

Print datasheet

Synonyms

EOMES, TBR2, 241036D9, T-box brain protein 2, T brain 2

验证数据展示

  • WB analysis using 83945-5-RR

    WB analysis using 83945-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83945-5-RR (EOMES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 83945-5-RR

    IF Staining of SH-SY5Y using 83945-5-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using EOMES antibody (83945-5-RR, Clone: 241036D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83945-5-RR

    Affinity and Kinetic Characterization of 83945-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human EOMES were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inNK-92 cells
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83945-5-RR targets EOMES/TBR2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen EOMES/TBR2 fusion protein Ag27634 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eomesodermin homolog (Xenopus laevis)
Synonyms EOMES, TBR2, 241036D9, T-box brain protein 2, T brain 2
Calculated Molecular Weight 73 aa
Observed Molecular Weight 67 kDa
GenBank Accession NumberNM_001278182
Gene Symbol EOMES
Gene ID (NCBI) 8320
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95936
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EOMES/TRB2 (also named Eomesodermin/Tbr2) encode T-box transcription factor expressed highly in the intermediate progenitor stage of the developing neuron. During migration of the neurons, radial glia divide and migrate towards the surface of the cerebral cortex, there are three stages of cellular development: radial glia, intermediate progenitors, and postmitotic projection neurons. Radial glia express Pax6, while intermediate progenitor cells express Eomesodermin/Tbr2, and postmitotic projection neurons express Tbr1 (PMID:15634788; PMID:16320258). This process also called neurogenesis, and demonstrated that Eomesodermin/Tbr2 has been implicated in neurodevelopment. According to the mouse model, several articles showed that EOMES/TRB2 KO mice having proliferating cells decreased and having smaller upper cortical layers and a smaller sub ventricular zone in the brain (PMID:18940588). What's more, Eomesodermin/Tbr2 has also been found participating in immune response (PMID:20713880).

实验方案

Product Specific Protocols
WB protocol for EOMES/TBR2 antibody 83945-5-RRDownload protocol
IF protocol for EOMES/TBR2 antibody 83945-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols