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验证数据展示

  • Sandwich ELISA standard curve of MP00775-4

    Sandwich ELISA standard curve of MP00775-4

    Sandwich ELISA standard curve of MP00775-4, Human HIF2α/EPAS1 Recombinant Matched Antibody Pair - PBS only. 83790-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15199. 83790-5-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • Sample test of MP00775-4

    Sample test of MP00775-4

    A549 and Hela cell lysates were measured. The human HIF2α/EPAS1 concentration of detected samples was determined to be 7,062.3 pg/mL (based on a 1.00 mg/mL extract load) in A549 cell lysate and 6,988.6 pg/mL (based on a 1.50 mg/mL extract load) in Hela cell lysate.

  • Cytometric bead array standard curve of MP00775-1

    Cytometric bead array standard curve of MP00775-1

    Cytometric bead array standard curve of MP00775-1, HIF2α/EPAS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83790-4-PBS. Detection antibody: 83790-1-PBS. Standard: Ag15199. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00775-3

    Cytometric bead array standard curve of MP00775-3

    Cytometric bead array standard curve of MP00775-3, HIF2α/EPAS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83790-4-PBS. Detection antibody: 83790-3-PBS. Standard: Ag15199. Range: 1.56-100 ng/mL.

HIF2α/EPAS1 Recombinant antibody, PBS Only (Capture)

HIF2α/EPAS1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83790-4-PBS

产品说明书

CloneNo. 240736H7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

EPAS1, Endothelial PAS domain-containing protein 1, ECYT4, Class E basic helix-loop-helix protein 73, bHLHe73

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83790-4-PBS targets HIF2α/EPAS1 as part of a matched antibody pair:

MP00775-1: 83790-4-PBS capture and 83790-1-PBS detection (validated in Cytometric bead array)

MP00775-3: 83790-4-PBS capture and 83790-3-PBS detection (validated in Cytometric bead array)

MP00775-4: 83790-4-PBS capture and 83790-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 HIF2α/EPAS1 fusion protein Ag15199 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endothelial PAS domain protein 1
别名 EPAS1, Endothelial PAS domain-containing protein 1, ECYT4, Class E basic helix-loop-helix protein 73, bHLHe73
计算分子量 96 kDa
GenBank蛋白编号BC051338
基因名称 HIF2α
Gene ID (NCBI) 2034
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99814
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C