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EPB41L1 Polyclonal antibody

EPB41L1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 13440-1-AP

Print datasheet

Synonyms

4.1N, Band 4.1 like protein 1, EPB41L1, KIAA0338, Neuronal protein 4.1

验证数据展示

  • WB analysis using 13440-1-AP

    WB analysis using 13440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13440-1-AP (EPB41L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 13440-1-AP

    WB analysis of mouse brain using 13440-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13440-1-AP (EPB41L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13440-1-AP

    IHC staining of mouse brain using 13440-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13440-1-AP (EPB41L1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13440-1-AP targets EPB41L1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen EPB41L1 fusion protein Ag4250 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name erythrocyte membrane protein band 4.1-like 1
Synonyms 4.1N, Band 4.1 like protein 1, EPB41L1, KIAA0338, Neuronal protein 4.1
Calculated Molecular Weight 880 aa, 98 kDa
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberBC040259
Gene Symbol EPB41L1
Gene ID (NCBI) 2036
RRIDAB_2877948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4G0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EPB41L1 antibody 13440-1-APDownload protocol
IHC protocol for EPB41L1 antibody 13440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Heliyon

Galactose-decorated liver tumor-specific nanoliposomes incorporating selective BRD4-targeted PROTAC for hepatocellular carcinoma therapy.

Authors - Aishwarya Saraswat
View Article