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EPB41L3 Polyclonal antibody

EPB41L3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10719-1-AP

Print datasheet

Synonyms

Erythrocyte membrane protein band 4.1-like 3, DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3

验证数据展示

  • WB analysis using 10719-1-AP

    WB analysis using 10719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10719-1-AP (EPB41L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 10719-1-AP

    IHC staining of human brain using 10719-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10719-1-AP (EPB41L3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L3 antibody included
  • IHC staining of human brain using 10719-1-AP

    IHC staining of human brain using 10719-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10719-1-AP (EPB41L3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L3 antibody included

经过测试的应用

Positive WB detected inPC-3 cells, U2OS cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EPB41L3 Ready-To-Use IHC Kit. EPB41L3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10719-1-AP targets EPB41L3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen EPB41L3 fusion protein Ag1089 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name erythrocyte membrane protein band 4.1-like 3
Synonyms Erythrocyte membrane protein band 4.1-like 3, DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3
Calculated Molecular Weight 121 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC008377
Gene Symbol EPB41L3
Gene ID (NCBI) 23136
RRIDAB_2098488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2J2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).

实验方案

Product Specific Protocols
WB protocol for EPB41L3 antibody 10719-1-APDownload protocol
IHC protocol for EPB41L3 antibody 10719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

The Role of Erythrocyte Membrane Protein Band 4.1-like 3 in Idiopathic Pulmonary Fibrosis

Authors - Min Kyung Kim
View Article