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验证数据展示

  • WB analysis using 10719-1-AP

    WB analysis using 10719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10719-1-AP (EPB41L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 10719-1-AP

    IHC staining of human brain using 10719-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10719-1-AP (EPB41L3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L3 antibody included
  • IHC staining of human brain using 10719-1-AP

    IHC staining of human brain using 10719-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10719-1-AP (EPB41L3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPB41L3 antibody included

EPB41L3 Polyclonal antibody

EPB41L3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10719-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Erythrocyte membrane protein band 4.1-like 3, DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, U2OS cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EPB41L3 Ready-To-Use IHC Kit. EPB41L3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10719-1-AP targets EPB41L3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 EPB41L3 fusion protein Ag1089 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 erythrocyte membrane protein band 4.1-like 3
别名 Erythrocyte membrane protein band 4.1-like 3, DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3
计算分子量 121 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC008377
基因名称 EPB41L3
Gene ID (NCBI) 23136
RRIDAB_2098488
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2J2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).

实验方案

Product Specific Protocols
WB protocol for EPB41L3 antibody 10719-1-APDownload protocol
IHC protocol for EPB41L3 antibody 10719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

The Role of Erythrocyte Membrane Protein Band 4.1-like 3 in Idiopathic Pulmonary Fibrosis

Authors - Min Kyung Kim
View Article