验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29180-1-AP targets EPB41L5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | EPB41L5 fusion protein Ag30300 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | erythrocyte membrane protein band 4.1 like 5 |
| 别名 | KIAA1548, Band 4.1 like protein 5 |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC032822 |
| 基因名称 | EPB41L5 |
| Gene ID (NCBI) | 57669 |
| RRID | AB_3669676 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HCM4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EPB41L5, a member of the NBL4 subgroup of the band 4.1 superfamily, contains a FERM domain and plays an important role in regulating the connection between plasma membrane proteins and the cytoskeleton underneath the plasma membrane (PMID: 30082297). EPB41L5 is a mesenchymal-specific protein induced during EMT (epithelial-mesenchymal transition) that promotes the disruption of cell-cell adhesion kinetics (PMID: 34784520). EPB41L5 exists as four alternatively spliced isoforms encoded by a gene located on human chromosome 2q14.2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPB41L5 antibody 29180-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |