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验证数据展示

  • IHC staining of human placenta using 27443-1-AP

    IHC staining of human placenta using 27443-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27443-1-AP (EPB42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 27443-1-AP

    IHC staining of human placenta using 27443-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27443-1-AP (EPB42 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27443-1-AP

    WB analysis using 27443-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27443-1-AP (EPB42 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EPB42 Polyclonal antibody

EPB42 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27443-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SPH5, Protein 4.2, PA, P4.2, Erythrocyte protein 4.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27443-1-AP targets EPB42 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EPB42 fusion protein Ag23556 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 erythrocyte membrane protein band 4.2
别名 SPH5, Protein 4.2, PA, P4.2, Erythrocyte protein 4.2
观测分子量72 kDa
GenBank蛋白编号BC096093
基因名称 EPB42
Gene ID (NCBI) 2038
RRIDAB_3085962
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16452
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.

实验方案

Product Specific Protocols
WB protocol for EPB42 antibody 27443-1-APDownload protocol
IHC protocol for EPB42 antibody 27443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols