• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

    WB analysis of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84921-3-RR (EPB42 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

    IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

    IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

  • IF Staining of HEL using 84921-3-RR (same clone as 84921-3-PBS)

    IF Staining of HEL using 84921-3-RR (same clone as 84921-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using EPB42 antibody (84921-3-RR, Clone: 242412F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01708-1

    Cytometric bead array standard curve of MP01708-1

    Cytometric bead array standard curve of MP01708-1, EPB42 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84921-2-PBS. Detection antibody: 84921-3-PBS. Standard: Ag23556. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01708-2

    Cytometric bead array standard curve of MP01708-2

    Cytometric bead array standard curve of MP01708-2, EPB42 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84921-1-PBS. Detection antibody: 84921-3-PBS. Standard: Ag23556. Range: 1.25-80 ng/mL.

EPB42 Recombinant antibody, PBS Only (Detector)

EPB42 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84921-3-PBS

产品说明书

CloneNo. 242412F4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

242412F4, E42P, Erythrocyte protein 4.2, P4.2, PA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84921-3-PBS targets EPB42 as part of a matched antibody pair:

MP01708-1: 84921-2-PBS capture and 84921-3-PBS detection (validated in Cytometric bead array)

MP01708-2: 84921-1-PBS capture and 84921-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 EPB42 fusion protein Ag23556 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 erythrocyte membrane protein band 4.2
别名 242412F4, E42P, Erythrocyte protein 4.2, P4.2, PA
观测分子量77 kDa
GenBank蛋白编号BC096093
基因名称 EPB42
Gene ID (NCBI) 2038
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16452
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.