验证数据展示
经过测试的应用
| Positive WB detected in | human red blood cells, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16808-1-AP targets Dematin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Dematin fusion protein Ag10300 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | erythrocyte membrane protein band 4.9 (dematin) |
| 别名 | EPB49, EPB 49, DMTN, Dematin actin-binding protein |
| 计算分子量 | 405 aa, 46 kDa |
| 观测分子量 | 48-52 kDa |
| GenBank蛋白编号 | BC017445 |
| 基因名称 | Dematin |
| Gene ID (NCBI) | 2039 |
| RRID | AB_2277891 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08495 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dematin (band 4.9) is a widely expressed cytoskeletal protein which regulates cell shape and cell migration. It is best known for its role within the red blood cell (RBC), where it has been localized to the spectrin-actin junctions of RBC membrane. Dematin has a 48 kDa subunit containing a headpiece domain and a 52 kDa subunit.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Dematin antibody 16808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Med Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome | ||
Exp Cell Res Dematin inhibits glioblastoma malignancy through RhoA-mediated CDKs downregulation and cytoskeleton remodeling. |