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CoraLite® Plus 488-conjugated EPCAM/CD326 Monoclonal antibody

EPCAM/CD326 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A2D5

Cat No : CL488-66316

Print datasheet

Synonyms

CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1

验证数据展示

  • IF Staining of HT-29 using CL488-66316

    IF Staining of HT-29 using CL488-66316

    Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using CoraLite® Plus 488 EPCAM/CD326 antibody (CL488-66316, Clone: 2A2D5 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHT-29 cells, MCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66316-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

CL488-66316 targets EPCAM/CD326 in WB, IF applications and shows reactivity with human samples.

Tested Applications IF/ICC
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen EPCAM/CD326 fusion protein Ag15393 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name epithelial cell adhesion molecule
Synonyms CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014785
Gene Symbol EPCAM
Gene ID (NCBI) 4072
RRIDAB_2919310
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16422
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. (PMID: 20837599; 19249674; 21576002; 22647938)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EPCAM/CD326 antibody CL488-66316Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Discov

MCM2 promotes the stemness and sorafenib resistance of hepatocellular carcinoma cells via hippo signaling

Authors - Xin Zhou
View Article