- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated EPCAM/CD326 Monoclonal antibody
EPCAM/CD326 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF/ICC and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A2D5
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HT-29 cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66316-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
CL488-66316 targets EPCAM/CD326 in WB, IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | EPCAM/CD326 fusion protein Ag15393 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | epithelial cell adhesion molecule |
| Synonyms | EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326 |
| Calculated Molecular Weight | 314 aa, 35 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC014785 |
| Gene Symbol | EPCAM |
| Gene ID (NCBI) | 4072 |
| RRID | AB_2919310 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16422 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. (PMID: 20837599; 19249674; 21576002; 22647938)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 EPCAM/CD326 antibody CL488-66316 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Discov MCM2 promotes the stemness and sorafenib resistance of hepatocellular carcinoma cells via hippo signaling |
