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EPCAM/CD326 Recombinant antibody, PBS Only

EPCAM/CD326 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241243B10

Cat no : 84073-6-PBS

Print datasheet

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

验证数据展示

  • WB analysis using 84073-6-RR (same clone as 84073-6-PBS)

    WB analysis using 84073-6-RR (same clone as 84073-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

    IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPCAM/CD326 antibody included
  • IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

    IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPCAM/CD326 antibody included
  • IF Staining of A431 using 84073-6-RR (same clone as 84073-6-PBS)

    IF Staining of A431 using 84073-6-RR (same clone as 84073-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EPCAM/CD326 antibody (84073-6-RR, Clone: 241243B10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EPCAM/CD326 Ready-To-Use IHC Kit. EPCAM/CD326 primary antibody included.

产品信息

The immunogen of 84073-6-PBS is EPCAM/CD326 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name epithelial cell adhesion molecule
Synonyms EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326
Calculated Molecular Weight35 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberNM_002354.3
Gene Symbol EPCAM
Gene ID (NCBI) 4072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16422
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)