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验证数据展示

  • WB analysis of A549 using 18698-1-AP

    WB analysis of A549 using 18698-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18698-1-AP (EPHA1-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 18698-1-AP

    IHC staining of human liver cancer using 18698-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18698-1-AP (EPHA1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 18698-1-AP

    IHC staining of human liver cancer using 18698-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18698-1-AP (EPHA1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 18698-1-AP

    IF Staining of A549 using 18698-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 18698-1-AP (EPHA1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

EPHA1-specific Polyclonal antibody

EPHA1-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18698-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

EPHA1, EPHA1-special, EPH tyrosine kinase 1, EPH tyrosine kinase, EPH receptor A1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18698-1-AP targets EPHA1-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EPH receptor A1
别名 EPHA1, EPHA1-special, EPH tyrosine kinase 1, EPH tyrosine kinase, EPH receptor A1
计算分子量 108 kDa
观测分子量 150 kDa
GenBank蛋白编号BC130291
基因名称 EPHA1
Gene ID (NCBI) 2041
RRIDAB_10597245
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21709
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.

实验方案

Product Specific Protocols
WB protocol for EPHA1-specific antibody 18698-1-APDownload protocol
IHC protocol for EPHA1-specific antibody 18698-1-APDownload protocol
IF protocol for EPHA1-specific antibody 18698-1-APDownload protocol
FC protocol for EPHA1-specific antibody 18698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols