验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18698-1-AP targets EPHA1-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | EPH receptor A1 |
别名 | EPHA1, EPHA1-special, EPH tyrosine kinase 1, EPH tyrosine kinase, EPH receptor A1 |
计算分子量 | 108 kDa |
观测分子量 | 150 kDa |
GenBank蛋白编号 | BC130291 |
基因名称 | EPHA1 |
Gene ID (NCBI) | 2041 |
RRID | AB_10597245 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P21709 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.
实验方案
Product Specific Protocols | |
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WB protocol for EPHA1-specific antibody 18698-1-AP | Download protocol |
IHC protocol for EPHA1-specific antibody 18698-1-AP | Download protocol |
IF protocol for EPHA1-specific antibody 18698-1-AP | Download protocol |
FC protocol for EPHA1-specific antibody 18698-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |