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验证数据展示

  • IHC staining of mouse brain using 55215-1-AP

    IHC staining of mouse brain using 55215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55215-1-AP

    IHC staining of mouse brain using 55215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 55215-1-AP

    IF Staining of SH-SY5Y using 55215-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 55215-1-AP (EPHA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

EPHA5 Polyclonal antibody

EPHA5 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 55215-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

CEK7, EHK 1, EHK1, EK7, EPH homology kinase 1, EPH like kinase 7, EPH receptor A5, EPHA5, Ephrin type A receptor 5, HEK7, TYRO4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55215-1-AP targets EPHA5 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EPH receptor A5
别名 CEK7, EHK 1, EHK1, EK7, EPH homology kinase 1, EPH like kinase 7, EPH receptor A5, EPHA5, Ephrin type A receptor 5, HEK7, TYRO4
计算分子量 115 kDa
GenBank蛋白编号NM_004439
基因名称 EPHA5
Gene ID (NCBI) 2044
RRIDAB_2881289
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54756
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for EPHA5 antibody 55215-1-APDownload protocol
IF protocol for EPHA5 antibody 55215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols