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验证数据展示

  • IHC staining of mouse brain using 55215-1-AP

    IHC staining of mouse brain using 55215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55215-1-AP

    IHC staining of mouse brain using 55215-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55215-1-AP (EPHA5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 55215-1-AP

    IF Staining of SH-SY5Y using 55215-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 55215-1-AP (EPHA5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

EPHA5 Polyclonal antibody

EPHA5 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 55215-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

CEK7, EHK 1, EHK1, EK7, EPH homology kinase 1, EPH like kinase 7, EPH receptor A5, EPHA5, Ephrin type A receptor 5, HEK7, TYRO4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55215-1-AP targets EPHA5 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EPH receptor A5
Synonyms CEK7, EHK 1, EHK1, EK7, EPH homology kinase 1, EPH like kinase 7, EPH receptor A5, EPHA5, Ephrin type A receptor 5, HEK7, TYRO4
Calculated Molecular Weight 115 kDa
GenBank Accession NumberNM_004439
Gene Symbol EPHA5
Gene ID (NCBI) 2044
RRIDAB_2881289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54756
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for EPHA5 antibody 55215-1-APDownload protocol
IF protocol for EPHA5 antibody 55215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols