验证数据展示
经过测试的应用
Positive WB detected in | mouse cerebellum tissue, mouse brain tissue, fetal human brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
67080-1-Ig targets EPHB1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | Human, Mouse |
文献引用反应性 | rat |
免疫原 | EPHB1 fusion protein Ag16347 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | EPH receptor B1 |
别名 | EK6, ELK, EPH like kinase 6, EPH receptor B1, EPHB1, Ephrin type B receptor 1, EPHT2, FLJ37986, Hek6, NET |
计算分子量 | 984 aa, 110 kDa |
观测分子量 | 110-120 kDa |
GenBank蛋白编号 | BC111744 |
基因名称 | EPHB1 |
Gene ID (NCBI) | 2047 |
RRID | AB_2882388 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P54762 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EPHB1, also named as EPHT2, NET, HEK6 and ELK, belongs to the protein kinase superfamily, Tyr protein kinase family, and Ephrin receptor subfamily. It is a receptor for members of the ephrin-B family. EPHB1 binds to ephrin-B1, -B2 and -B3. EPHB1 may be involved in cell-cell interactions in the nervous system. EPHB1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
实验方案
Product Specific Protocols | |
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WB protocol for EPHB1 antibody 67080-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |