• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of mouse brain using 21382-1-AP

    IHC staining of mouse brain using 21382-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21382-1-AP (EPHB3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21382-1-AP

    IHC staining of mouse brain using 21382-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21382-1-AP (EPHB3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPHB3 Polyclonal antibody

EPHB3 Polyclonal Antibody for IHC, ELISA
Cat No. 21382-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

HEK2, ETK2, Ephrin type B receptor 3, EPH receptor B3, EPH like kinase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21382-1-AP targets EPHB3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 EPHB3 fusion protein Ag16016 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EPH receptor B3
别名 HEK2, ETK2, Ephrin type B receptor 3, EPH receptor B3, EPH like kinase 2
计算分子量 998 aa, 110 kDa
观测分子量90-100 kDa
GenBank蛋白编号BC052968
基因名称 EPHB3
Gene ID (NCBI) 2049
RRIDAB_3669366
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54753
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPHB3 is a member of the Eph receptor tyrosine kinase family. The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). The EPHB3 protein has multiple functions in the nervous system, including regulating neural networks, participating in epileptic activity, and playing a role in repair after spinal cord injury.

实验方案

Product Specific Protocols
IHC protocol for EPHB3 antibody 21382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols