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  • Featured Product
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EPHX2 Polyclonal antibody

EPHX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10833-1-AP

Print datasheet

Synonyms

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

验证数据展示

  • WB analysis of HEK-293 using 10833-1-AP

    WB analysis of HEK-293 using 10833-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10833-1-AP (EPHX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 10833-1-AP

    WB analysis of A549 using 10833-1-AP

    WB result of EPHX2 antibody (10833-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells.

  • IP experiment of mouse large intestine using 10833-1-AP

    IP experiment of mouse large intestine using 10833-1-AP

    IP result of anti-EPHX2 (IP:10833-1-AP, 4ug; Detection:10833-1-AP 1:300) with mouse large intestine tissue lysate 3200 ug.

  • IHC staining of human colon cancer using 10833-1-AP

    IHC staining of human colon cancer using 10833-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10833-1-AP (EPHX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPHX2 antibody included
  • IHC staining of human colon cancer using 10833-1-AP

    IHC staining of human colon cancer using 10833-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10833-1-AP (EPHX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPHX2 antibody included
  • IHC staining of rat kidney using 10833-1-AP

    IHC staining of rat kidney using 10833-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPHX2 antibody included
  • IHC staining of mouse brain using 10833-1-AP

    IHC staining of mouse brain using 10833-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPHX2 antibody included
  • IHC staining of mouse kidney using 10833-1-AP

    IHC staining of mouse kidney using 10833-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10833-1-AP (EPHX2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPHX2 antibody included
  • IF Staining of HEK-293 using 10833-1-AP

    IF Staining of HEK-293 using 10833-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10833-1-AP (EPHX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse colon tissue, rat cerebellum tissue, HEK-293T cells, A549 cells
Positive IP detected inmouse large intestine tissue, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy EPHX2 Ready-To-Use IHC Kit. EPHX2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10833-1-AP targets EPHX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Immunogen EPHX2 fusion protein Ag1283 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epoxide hydrolase 2, cytoplasmic
Synonyms SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC013874
Gene Symbol EPHX2
Gene ID (NCBI) 2053
RRIDAB_2098246
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34913
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

实验方案

Product Specific Protocols
WB protocol for EPHX2 antibody 10833-1-APDownload protocol
IHC protocol for EPHX2 antibody 10833-1-APDownload protocol
IF protocol for EPHX2 antibody 10833-1-APDownload protocol
IP protocol for EPHX2 antibody 10833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Gasdermin D-mediated metabolic crosstalk promotes tissue repair

Authors - Zhexu Chi
View Article
mouseWB,IF

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang
View Article
humanWB

Int J Biol Sci

Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury

Authors - Juan Zhang
View Article
ratWB

Biochim Biophys Acta Mol Basis Dis

Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ

Authors - Yang Zhou
View Article
humanWB,IHC

Front Oncol

EPHX2 Inhibits Colon Cancer Progression by Promoting Fatty Acid Degradation.

Authors - Yiran Zhou
View Article
chickenWB

Arch Biochem Biophys

Mitochondrial P450-dependent arachidonic acid metabolism by TCDD-induced hepatic CYP1A5; conversion of EETs to DHETs by mitochondrial soluble epoxide hydrolase.

Authors - Labitzke Erin M EM
View Article