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EPHX2 Polyclonal antibody

EPHX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10833-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
Biotin
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, mouse colon tissue, rat cerebellum tissue, HEK-293T cells, A549 cells
Positive IP detected inmouse large intestine tissue, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy EPHX2 Ready-To-Use IHC Kit. EPHX2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10833-1-AP targets EPHX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, chicken
免疫原 EPHX2 fusion protein Ag1283 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epoxide hydrolase 2, cytoplasmic
别名 SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2
计算分子量 63 kDa
观测分子量 63 kDa
GenBank蛋白编号BC013874
基因名称 EPHX2
Gene ID (NCBI) 2053
RRIDAB_2098246
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP34913
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

实验方案

Product Specific Protocols
WB protocol for EPHX2 antibody 10833-1-APDownload protocol
IHC protocol for EPHX2 antibody 10833-1-APDownload protocol
IF protocol for EPHX2 antibody 10833-1-APDownload protocol
IP protocol for EPHX2 antibody 10833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Gasdermin D-mediated metabolic crosstalk promotes tissue repair

Authors - Zhexu Chi
mouseWB,IF

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang
humanWB

Int J Biol Sci

Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury

Authors - Juan Zhang
ratWB

Biochim Biophys Acta Mol Basis Dis

Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ

Authors - Yang Zhou
ratWB,IHC,IF

Eur J Pharmacol

Restoration of ARA metabolic disorders in vascular smooth muscle cells alleviates intimal hyperplasia

Authors - Hui Wu
humanWB,IHC

Front Oncol

EPHX2 Inhibits Colon Cancer Progression by Promoting Fatty Acid Degradation.

Authors - Yiran Zhou
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