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验证数据展示

  • WB analysis using 67322-1-Ig (same clone as 67322-1-PBS)

    WB analysis using 67322-1-Ig (same clone as 67322-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67322-1-Ig (EPHX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 67322-1-Ig (same clone as 67322-1-PBS)

    WB analysis of A549 using 67322-1-Ig (same clone as 67322-1-PBS)

    WB result of EPHX2 antibody (67322-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells. This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67322-1-Ig (same clone as 67322-1-PBS)

    IHC staining of human breast cancer using 67322-1-Ig (same clone as 67322-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 10x lens). This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPHX2 antibody included
  • IHC staining of human breast cancer using 67322-1-Ig (same clone as 67322-1-PBS)

    IHC staining of human breast cancer using 67322-1-Ig (same clone as 67322-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 40x lens). This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EPHX2 antibody included
  • IF Staining of A549 using 67322-1-Ig (same clone as 67322-1-PBS)

    IF Staining of A549 using 67322-1-Ig (same clone as 67322-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using EPHX2 antibody (67322-1-Ig, Clone: 4H6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EPHX2 Monoclonal antibody, PBS Only

EPHX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67322-1-PBS

产品说明书

CloneNo. 4H6C8

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EPHX2 Ready-To-Use IHC Kit. EPHX2 primary antibody included.

产品信息

67322-1-PBS targets EPHX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 EPHX2 fusion protein Ag4870 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 epoxide hydrolase 2, cytoplasmic
别名 SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2
计算分子量 63 kDa
观测分子量 56-63 kDa
GenBank蛋白编号BC013874
基因名称 EPHX2
Gene ID (NCBI) 2053
RRIDAB_2882582
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP34913
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).