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EPHX3 Polyclonal antibody

EPHX3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25608-1-AP

Print datasheet

Synonyms

EH3, EC:3.3.2.10, Abhydrolase domain-containing protein 9, ABHD9

验证数据展示

  • IHC staining of human breast cancer using 25608-1-AP

    IHC staining of human breast cancer using 25608-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25608-1-AP (EPHX3 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 25608-1-AP

    IF Staining of A549 using 25608-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EPHX3 antibody (25608-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25608-1-AP targets EPHX3 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen EPHX3 fusion protein Ag22277 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epoxide hydrolase 3
Synonyms EH3, EC:3.3.2.10, Abhydrolase domain-containing protein 9, ABHD9
Calculated Molecular Weight 360 aa, 41 kDa
GenBank Accession NumberBC132960
Gene Symbol EPHX3
Gene ID (NCBI) 79852
RRIDAB_2880158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6B9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for EPHX3 antibody 25608-1-APDownload protocol
IF protocol for EPHX3 antibody 25608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols