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  • Featured Product
  • KD/KO Validated

EPLIN Polyclonal antibody, PBS Only

EPLIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16639-1-PBS

Print datasheet

Synonyms

LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1

验证数据展示

  • WB analysis of HEK-293 using 16639-1-AP (same clone as 16639-1-PBS)

    WB analysis of HEK-293 using 16639-1-AP (same clone as 16639-1-PBS)

    WB result of EPLIN antibody (16639-1-AP, 1:1000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 16639-1-AP (same clone as 16639-1-PBS)

    IHC staining of human prostate cancer using 16639-1-AP (same clone as 16639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 16639-1-AP (same clone as 16639-1-PBS)

    IP experiment of mouse liver using 16639-1-AP (same clone as 16639-1-PBS)

    IP result of anti-EPLIN (IP:16639-1-AP, 4ug; Detection:16639-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 16639-1-AP (same clone as 16639-1-PBS)

    IF Staining of PC-3 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • WB analysis of HUVEC using 16639-1-AP (same clone as 16639-1-PBS)

    WB analysis of HUVEC using 16639-1-AP (same clone as 16639-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

    IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

产品信息

16639-1-PBS targets EPLIN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EPLIN fusion protein Ag9994 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LIM domain and actin binding 1
Synonyms LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90 kDa, 110 kDa
GenBank Accession NumberBC001247
Gene Symbol EPLIN
Gene ID (NCBI) 51474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHB6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues.