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验证数据展示

  • WB analysis using 14469-1-AP

    WB analysis using 14469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14469-1-AP (EPN1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14469-1-AP

    IF Staining of HeLa using 14469-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPN1 antibody (14469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

EPN1 Polyclonal antibody

EPN1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14469-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Epsin-1, EPS-15-interacting protein 1, EH domain-binding mitotic phosphoprotein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14469-1-AP targets EPN1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen EPN1 fusion protein Ag5838 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epsin 1
Synonyms Epsin-1, EPS-15-interacting protein 1, EH domain-binding mitotic phosphoprotein
Calculated Molecular Weight 60 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC044651
Gene Symbol EPN1
Gene ID (NCBI) 29924
RRIDAB_3085436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6I3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epsin 1 (EPN1) belongs to the epsin family, which also includes EPN2 and EPN3. They belong to the endocytic clathrin family and are involved in receptor endocytosis. EPN1 is widely and highly expressed in tumors.

实验方案

Product Specific Protocols
WB protocol for EPN1 antibody 14469-1-APDownload protocol
IF protocol for EPN1 antibody 14469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols