• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 14469-1-AP

    WB analysis using 14469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14469-1-AP (EPN1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14469-1-AP

    IF Staining of HeLa using 14469-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPN1 antibody (14469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

EPN1 Polyclonal antibody

EPN1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14469-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Epsin-1, EPS-15-interacting protein 1, EH domain-binding mitotic phosphoprotein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14469-1-AP targets EPN1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 EPN1 fusion protein Ag5838 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epsin 1
别名 Epsin-1, EPS-15-interacting protein 1, EH domain-binding mitotic phosphoprotein
计算分子量 60 kDa
观测分子量65 kDa
GenBank蛋白编号BC044651
基因名称 EPN1
Gene ID (NCBI) 29924
RRIDAB_3085436
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6I3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epsin 1 (EPN1) belongs to the epsin family, which also includes EPN2 and EPN3. They belong to the endocytic clathrin family and are involved in receptor endocytosis. EPN1 is widely and highly expressed in tumors.

实验方案

Product Specific Protocols
WB protocol for EPN1 antibody 14469-1-APDownload protocol
IF protocol for EPN1 antibody 14469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols