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验证数据展示

  • WB analysis using 31334-1-AP

    WB analysis using 31334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31334-1-AP (EPN2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 31334-1-AP

    IHC staining of mouse lung using 31334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31334-1-AP (EPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 31334-1-AP

    IHC staining of mouse lung using 31334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31334-1-AP (EPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPN2 Polyclonal antibody

EPN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31334-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KIAA1065, Epsin-2, epsin 2, EPS-15-interacting protein 2, EPS 15 interacting protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31334-1-AP targets EPN2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 EPN2 fusion protein Ag35194 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epsin 2
别名 KIAA1065, Epsin-2, epsin 2, EPS-15-interacting protein 2, EPS 15 interacting protein 2
计算分子量 584 aa, 62 kDa
观测分子量68 kDa
GenBank蛋白编号BC093972
基因名称 EPN2
Gene ID (NCBI) 22905
RRIDAB_3669948
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO95208
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPN2 (EPS 15 interacting protein 2) is a protein that interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. EPN2 is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery.

实验方案

Product Specific Protocols
WB protocol for EPN2 antibody 31334-1-APDownload protocol
IHC protocol for EPN2 antibody 31334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols