验证数据展示
经过测试的应用
| Positive WB detected in | MCF-10A cells, MCF-7 cells, mouse stomach tissue, rat stomach tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20178-1-AP targets EPN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | EPN3 fusion protein Ag14021 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | epsin 3 |
| 别名 | Epsin-3, EPS-15-interacting protein 3 |
| 计算分子量 | 632 aa, 68 kDa |
| 观测分子量 | 68-70 kDa |
| GenBank蛋白编号 | BC001038 |
| 基因名称 | EPN3 |
| Gene ID (NCBI) | 55040 |
| RRID | AB_3669327 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H201 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Epsin 3 (EPN3) belongs to the epsin family, which also includes EPN1 and EPN2. They belong to the endocytic clathrin family and are involved in receptor endocytosis. EPN3 is expressed in breast cancer and is an oncogene associated with breast cancer prognosis and treatment (PMID: 34779498;PMID: 32541686).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPN3 antibody 20178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
