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EPN3 Polyclonal antibody

EPN3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20178-1-AP

Print datasheet

Synonyms

Epsin-3, EPS-15-interacting protein 3

验证数据展示

  • WB analysis using 20178-1-AP

    WB analysis using 20178-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20178-1-AP (EPN3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-10A cells, MCF-7 cells, mouse stomach tissue, rat stomach tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20178-1-AP targets EPN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EPN3 fusion protein Ag14021 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epsin 3
Synonyms Epsin-3, EPS-15-interacting protein 3
Calculated Molecular Weight 632 aa, 68 kDa
Observed Molecular Weight68-70 kDa
GenBank Accession NumberBC001038
Gene Symbol EPN3
Gene ID (NCBI) 55040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H201
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epsin 3 (EPN3) belongs to the epsin family, which also includes EPN1 and EPN2. They belong to the endocytic clathrin family and are involved in receptor endocytosis. EPN3 is expressed in breast cancer and is an oncogene associated with breast cancer prognosis and treatment (PMID: 34779498;PMID: 32541686).

实验方案

Product Specific Protocols
WB protocol for EPN3 antibody 20178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols