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EPO/Erythropoietin Monoclonal antibody, PBS Only

EPO/Erythropoietin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11F3

Cat no : 66975-1-PBS

Print datasheet

Synonyms

EPO, MVCD2, erythropoietin, Epoetin, EP

验证数据展示

  • WB analysis of Recombinant protein using 66975-1-Ig (same clone as 66975-1-PBS)

    WB analysis of Recombinant protein using 66975-1-Ig (same clone as 66975-1-PBS)

    50 ng recombinant human EPO (cat.NO. HZ-1168) was subjected to SDS-PAGE under reducing condition followed by western blot with 66975-1-Ig (EPO antibody) at dilution of 1:10000. This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

产品信息

66975-1-PBS targets EPO/Erythropoietin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen EPO/Erythropoietin fusion protein Ag23054 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name erythropoietin
Synonyms EPO, MVCD2, erythropoietin, Epoetin, EP
Calculated Molecular Weight 193 aa, 21 kDa
GenBank Accession NumberBC093628
Gene Symbol EPO/Erythropoietin
Gene ID (NCBI) 2056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01588
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.