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验证数据展示

  • WB analysis using 30766-1-AP

    WB analysis using 30766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30766-1-AP (EPOR antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

EPOR Polyclonal antibody

EPOR Polyclonal Antibody for WB, ELISA
Cat No. 30766-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, ELISA

别名

EPO R, EPOR, erythropoietin receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse kidney tissue, mouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30766-1-AP targets EPOR in WB, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 EPOR fusion protein Ag33956 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 erythropoietin receptor
别名 EPO R, EPOR, erythropoietin receptor
计算分子量55 kDa
观测分子量70 kDa
GenBank蛋白编号NM_000121.4
基因名称 EPOR
Gene ID (NCBI) 2057
RRIDAB_3086414
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19235
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Erythropoietin (EPO) receptor (EPOR) is a glycoprotein that belongs to the type I superfamily of single-transmembrane cytokine receptors. It consists of an extracellular domain that binds to the EPO ligand, a transmembrane domain and an intracellular domain. The interaction of EPO and EPOR triggers the activation of several signaling pathways that induce erythropoiesis, including JAK2/STAT5, PI3K/AKT, and MAPK (PMID: 34281163). EPOR is present on erythroid progenitor cells and has also been detected on a wide variety of non-hematopoietic cells (PMID: 36233351). The calculated molecular weight of EPOR is 55 kDa. EPOR undergoes posttranslational modification and the apparent molecular weight of 66-78 kDa has been reported (PMID: 8943308; 12088257).

实验方案

Product Specific Protocols
WB protocol for EPOR antibody 30766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols