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EPOR Polyclonal antibody

EPOR Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30766-1-AP

Print datasheet

Synonyms

EPO R, EPOR, erythropoietin receptor

验证数据展示

  • WB analysis using 30766-1-AP

    WB analysis using 30766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30766-1-AP (EPOR antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman placenta tissue, mouse kidney tissue, mouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30766-1-AP targets EPOR in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen EPOR fusion protein Ag33956 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name erythropoietin receptor
Synonyms EPO R, EPOR, erythropoietin receptor
Calculated Molecular Weight55 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_000121.4
Gene Symbol EPOR
Gene ID (NCBI) 2057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19235
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Erythropoietin (EPO) receptor (EPOR) is a glycoprotein that belongs to the type I superfamily of single-transmembrane cytokine receptors. It consists of an extracellular domain that binds to the EPO ligand, a transmembrane domain and an intracellular domain. The interaction of EPO and EPOR triggers the activation of several signaling pathways that induce erythropoiesis, including JAK2/STAT5, PI3K/AKT, and MAPK (PMID: 34281163). EPOR is present on erythroid progenitor cells and has also been detected on a wide variety of non-hematopoietic cells (PMID: 36233351). The calculated molecular weight of EPOR is 55 kDa. EPOR undergoes posttranslational modification and the apparent molecular weight of 66-78 kDa has been reported (PMID: 8943308; 12088257).

实验方案

Product Specific Protocols
WB protocol for EPOR antibody 30766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols