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EPOR Polyclonal antibody

EPOR Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55308-1-AP

Print datasheet

Synonyms

EPO R, EPOR, erythropoietin receptor

验证数据展示

  • WB analysis of HepG2 using 55308-1-AP

    WB analysis of HepG2 using 55308-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55308-1-AP (EPOR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 55308-1-AP

    WB analysis of Jurkat using 55308-1-AP

    WB result of EPOR antibody (55308-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPOR transfected Jurkat cells.

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, mouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55308-1-AP targets EPOR in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name erythropoietin receptor
Synonyms EPO R, EPOR, erythropoietin receptor
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 66 kDa, 78 kDa
GenBank Accession NumberNM_000121
Gene Symbol EPOR
Gene ID (NCBI) 2057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19235
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Erythropoietin (EPO) receptor (EPOR) is a glycoprotein that belongs to the type I superfamily of single-transmembrane cytokine receptors. It consists of an extracellular domain that binds to the EPO ligand, a transmembrane domain and an intracellular domain. The interaction of EPO and EPOR triggers the activation of several signaling pathways that induce erythropoiesis, including JAK2/STAT5, PI3K/AKT, and MAPK (PMID: 34281163). EPOR is present on erythroid progenitor cells and has also been detected on a wide variety of non-hematopoietic cells (PMID: 36233351). The calculated molecular weight of EPOR is 55 kDa. EPOR undergoes posttranslational modification and the apparent molecular weight of 66-78 kDa has been reported (PMID: 8943308; 12088257).

实验方案

Product Specific Protocols
WB protocol for EPOR antibody 55308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PeerJ

RNA N6-methyladenosine reader IGF2BP3 promotes acute myeloid leukemia progression by controlling stabilization of EPOR mRNA

Authors - Jin Fan
View Article