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EPRS Polyclonal antibody

EPRS Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25307-1-AP

Print datasheet

Synonyms

EPRS1, GLNS, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase

验证数据展示

  • WB analysis using 25307-1-AP

    WB analysis using 25307-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25307-1-AP (EPRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 25307-1-AP

    IF Staining of HEK-293 using 25307-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25307-1-AP (EPRS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy EPRS Ready-To-Use IHC Kit. EPRS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

25307-1-AP targets EPRS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen EPRS fusion protein Ag18011 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamyl-prolyl-tRNA synthetase
Synonyms EPRS1, GLNS, EARS, Cell proliferation-inducing gene 32 protein, Bifunctional glutamate/proline--tRNA ligase
Calculated Molecular Weight 1512 aa, 171 kDa
Observed Molecular Weight 171 kDa
GenBank Accession NumberBC126275
Gene Symbol EPRS
Gene ID (NCBI) 2058
RRIDAB_2880023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07814
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)

实验方案

Product Specific Protocols
WB protocol for EPRS antibody 25307-1-APDownload protocol
IF protocol for EPRS antibody 25307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proteomics

Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.

Authors - Jing Zhang
View Article
humanWB

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj
View Article