验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy EPS15 Ready-To-Use IHC Kit. EPS15 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31301-1-AP targets EPS15 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | EPS15 fusion protein Ag35282 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | epidermal growth factor receptor pathway substrate 15 |
| 别名 | AF 1P, AF1P, EPS15, MLLT5, Protein AF 1p, Protein Eps15 |
| 计算分子量 | 99 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | NM_001981.3 |
| 基因名称 | EPS15 |
| Gene ID (NCBI) | 2060 |
| RRID | AB_3669938 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P42566 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EPS15 was originally identified as a substrate for the kinase activity of the epidermal growth factor receptor (EGFR). EPS15 has a tripartite structure comprising an NH2-terminal portion, which contains three EH domains, a central putative coiled-coil region, and a COOH-terminal domain containing multiple copies of the amino acid triplet Aspartate-Proline-Phenylalanine (PMID: 10481267). EPS15 is phosphorylated on tyrosine residues following EGFR activation (PMID: 7689153).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EPS15 antibody 31301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |