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验证数据展示

  • WB analysis using 12455-1-AP

    WB analysis using 12455-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12455-1-AP (EPS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12455-1-AP

    IP experiment of HeLa using 12455-1-AP

    IP result of anti-EPS8 (IP:12455-1-AP, 3ug; Detection:12455-1-AP 1:500) with HeLa cells lysate 3800ug.

  • IF Staining of A375 using 12455-1-AP

    IF Staining of A375 using 12455-1-AP

    Immunofluorescent analysis of A375 cells using 12455-1-AP (EPS8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human breast cancer using 12455-1-AP

    IHC staining of human breast cancer using 12455-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12455-1-AP (EPS8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPS8 antibody included
  • IHC staining of human breast cancer using 12455-1-AP

    IHC staining of human breast cancer using 12455-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12455-1-AP (EPS8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EPS8 antibody included
  • WB analysis of HeLa using 12455-1-AP

    WB analysis of HeLa using 12455-1-AP

    WB result of EPS8 antibody (12455-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS8 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

EPS8 Polyclonal antibody

EPS8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12455-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy EPS8 Ready-To-Use IHC Kit. EPS8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12455-1-AP targets EPS8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen EPS8 fusion protein Ag3121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epidermal growth factor receptor pathway substrate 8
Synonyms
Calculated Molecular Weight 822 aa, 92 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC030010
Gene Symbol EPS8
Gene ID (NCBI) 2059
RRIDAB_2098836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12929
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.

实验方案

Product Specific Protocols
WB protocol for EPS8 antibody 12455-1-APDownload protocol
IHC protocol for EPS8 antibody 12455-1-APDownload protocol
IF protocol for EPS8 antibody 12455-1-APDownload protocol
IP protocol for EPS8 antibody 12455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Exp Cell Res

EPS8 supports pancreatic cancer growth by inhibiting BMI1 mediated proteasomal degradation of ALDH7A1.

Authors - Mingzhu Tan
  • KD Validated
View Article
humanWB

Acta Biochim Biophys Sin (Shanghai)

miR-345 inhibits migration and stem-like cell phenotype in gastric cancer via inactivation of Rac1 by targeting EPS8.

Authors - Jieyun Zhang
  • KD Validated
View Article