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验证数据展示

  • WB analysis of A431 using 68330-1-Ig

    WB analysis of A431 using 68330-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68330-1-Ig

    WB analysis of HepG2 using 68330-1-Ig

    WB result of EPS8L2 antibody (68330-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS8L2 transfected HepG2 cells.

  • IF Staining of HeLa using 68330-1-Ig

    IF Staining of HeLa using 68330-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPS8L2 antibody (68330-1-Ig, Clone: 2E3B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of rat skin using 68330-1-Ig

    WB analysis of rat skin using 68330-1-Ig

    rat skin tissue were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

EPS8L2 Monoclonal antibody

EPS8L2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68330-1-Ig

产品说明书

CloneNo. 2E3B1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, ELISA

别名

EPS8 like 2, EPS8 like protein 2, EPS8 related protein 2, EPS8L2, EPS8R2, FLJ16738, FLJ21935, FLJ22171

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat skin tissue, A431 cells, HepG2 cells, A549 cells，LNCaP cells，HeLa cells，HepG2 cells, mouse skin tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68330-1-Ig targets EPS8L2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 EPS8L2 fusion protein Ag16808 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 EPS8-like 2
别名 EPS8 like 2, EPS8 like protein 2, EPS8 related protein 2, EPS8L2, EPS8R2, FLJ16738, FLJ21935, FLJ22171
计算分子量 715 aa, 81 kDa
观测分子量81-85 kDa
GenBank蛋白编号BC002474
基因名称 EPS8L2
Gene ID (NCBI) 64787
RRIDAB_2935402
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H6S3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPS8L2 (epidermal growth factor receptor pathway substrate 8 L2), a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells.

实验方案

Product Specific Protocols
WB protocol for EPS8L2 antibody 68330-1-IgDownload protocol
IF protocol for EPS8L2 antibody 68330-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols