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验证数据展示

  • WB analysis using 29755-1-AP

    WB analysis using 29755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29755-1-AP (EPX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 29755-1-AP

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 29755-1-AP

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPX Polyclonal antibody

EPX Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29755-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, pig, mouse

应用

WB, IHC, ELISA

别名

eosinophil peroxidase, EPER, EPO, EPP, EPX, EPX PEN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig bone marrow tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29755-1-AP targets EPX in WB, IHC, ELISA applications and shows reactivity with Human, pig, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, pig, mouse
免疫原 EPX fusion protein Ag30561 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eosinophil peroxidase
别名 eosinophil peroxidase, EPER, EPO, EPP, EPX, EPX PEN
计算分子量 81 kDa
观测分子量55 kDa
GenBank蛋白编号NM_000502
基因名称 EPX
Gene ID (NCBI) 8288
RRIDAB_3086154
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11678
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EPX antibody 29755-1-APDownload protocol
IHC protocol for EPX antibody 29755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols