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验证数据展示

  • WB analysis using 13643-1-AP

    WB analysis using 13643-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13643-1-AP (AHSP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13643-1-AP

    IHC staining of human placenta using 13643-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 13643-1-AP

    IHC staining of mouse lung using 13643-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AHSP Polyclonal antibody

AHSP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13643-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig

应用

WB, IHC, ELISA

别名

ERAF, Erythroid-associated factor, Erythroid differentiation-related factor, erythroid associated factor, EDRF

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig bone marrow tissue
Positive IHC detected inhuman placenta tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13643-1-AP targets AHSP in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, pig
免疫原

CatNo: Ag4588

Product name: Recombinant human ERAF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-102 aa of BC035842

Sequence: MALLKANKDLISAGLKEFSVLLNQQVFNDPLVSEEDMVTVVEDWMNFYINYYRQQVTGEPQERDKALQELRQELNTLANPFLAKYRDFLKSHELPSHPPPSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 erythroid associated factor
别名 ERAF, Erythroid-associated factor, Erythroid differentiation-related factor, erythroid associated factor, EDRF
计算分子量 102 aa, 12 kDa
观测分子量 10 kDa
GenBank蛋白编号BC035842
基因名称 AHSP
Gene ID (NCBI) 51327
RRIDAB_3669167
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZD4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development, and specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia (PMID: 12066189).

实验方案

Product Specific Protocols
IHC protocol for AHSP antibody 13643-1-APDownload protocol
WB protocol for AHSP antibody 13643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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