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验证数据展示

  • WB analysis of HEK-293T using 29465-1-AP

    WB analysis of HEK-293T using 29465-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 29465-1-AP (ERAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 29465-1-AP

    IHC staining of human colon using 29465-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ERAL1 antibody included
  • IHC staining of human colon using 29465-1-AP

    IHC staining of human colon using 29465-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ERAL1 antibody included

ERAL1 Polyclonal antibody

ERAL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29465-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

CEGA, Conserved ERA like GTPase, ERA, Era G protein like 1 (E. coli), ERA W, ERAL1, ERAL1A, hERA, HERA A, HERA B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, K-562 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ERAL1 Ready-To-Use IHC Kit. ERAL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29465-1-AP targets ERAL1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 ERAL1 fusion protein Ag30720 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Era G-protein-like 1 (E. coli)
别名 CEGA, Conserved ERA like GTPase, ERA, Era G protein like 1 (E. coli), ERA W, ERAL1, ERAL1A, hERA, HERA A, HERA B
计算分子量 437 aa, 48 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC019094
基因名称 ERAL1
Gene ID (NCBI) 26284
RRIDAB_2918311
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75616
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. ERAL1 localizes to the mitochondrionmitoribosomal subunit, and binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of ERAL1 has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis.

实验方案

Product Specific Protocols
WB protocol for ERAL1 antibody 29465-1-APDownload protocol
IHC protocol for ERAL1 antibody 29465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols