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验证数据展示

  • WB analysis of K-562 using 13821-1-AP

    WB analysis of K-562 using 13821-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13821-1-AP (ARTS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 13821-1-AP

    IHC staining of human ovary tumor using 13821-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13821-1-AP (ARTS1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human ovary tumor using 13821-1-AP

    IHC staining of human ovary tumor using 13821-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13821-1-AP (ARTS1 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of Jurkat using 13821-1-AP

    IP experiment of Jurkat using 13821-1-AP

    IP result of anti-ARTS1 (IP:13821-1-AP, 4ug; Detection:13821-1-AP 1:600) with Jurkat cells lysate 3600ug.

ARTS1 Polyclonal antibody

ARTS1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13821-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

A LAP, ALAP, Aminopeptidase PILS, APPILS, ARTS 1, ARTS1, ERAAP, ERAAP1, ERAP1, KIAA0525, PILS AP, PILSAP, Puromycin insensitive leucyl specific aminopeptidase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13821-1-AP targets ARTS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ARTS1 fusion protein Ag4918 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endoplasmic reticulum aminopeptidase 1
别名 A LAP, ALAP, Aminopeptidase PILS, APPILS, ARTS 1, ARTS1, ERAAP, ERAAP1, ERAP1, KIAA0525, PILS AP, PILSAP, Puromycin insensitive leucyl specific aminopeptidase
计算分子量 107 kDa
观测分子量 106 kDa
GenBank蛋白编号BC030775
基因名称 ARTS1/ERAP1
Gene ID (NCBI) 51752
RRIDAB_2293531
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ08
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARTS1 is an endoplasmic reticulum-associated aminopeptidase (also known as ERAP1). ERAP1 is induced by interferon-gama and can degrade peptides 9-15 residues long(PMID:18987748).ARTS1 is a 100 kDa, monomeric, soluble zinc aminopeptidase. It can be glycosylated to 120KDa and form homodimers and heterodimers(PMID:15908954).

实验方案

Product Specific Protocols
WB protocol for ARTS1 antibody 13821-1-APDownload protocol
IHC protocol for ARTS1 antibody 13821-1-APDownload protocol
IP protocol for ARTS1 antibody 13821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

ERAP1-ERAP2 Dimerization Increases Peptide-Trimming Efficiency.

Authors - Irini Evnouchidou
View Article

Autoimmunity

Endoplasmic reticulum aminopeptidase-1 alleles associated with increased risk of ankylosing spondylitis reduce HLA-B27 mediated presentation of multiple antigens.

Authors - Seregin Sergey S SS
View Article