• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84046-1-RR (same clone as 84046-1-PBS)

    WB analysis using 84046-1-RR (same clone as 84046-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84046-1-RR (ERBB2,p185-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 84046-1-RR (same clone as 84046-1-PBS)

    IHC staining of human ovarian cancer using 84046-1-RR (same clone as 84046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84046-1-RR (HER2/ErbB2,p185-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse uterus using 84046-1-RR (same clone as 84046-1-PBS)

    IHC staining of mouse uterus using 84046-1-RR (same clone as 84046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 84046-1-RR (HER2/ErbB2,p185-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 84046-1-RR (same clone as 84046-1-PBS)

    IF Staining of MCF-7 using 84046-1-RR (same clone as 84046-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HER2/ErbB2,p185-Specific antibody (84046-1-RR, Clone: 240802C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

HER2/ErbB2,p185-Specific Recombinant antibody, PBS Only

HER2/ErbB2,p185-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84046-1-PBS

产品说明书

CloneNo. 240802C2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ERBB2, HER2, HER 2/neu, HER 2, CD340

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84046-1-PBS targets HER2/ErbB2,p185-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
别名 ERBB2, HER2, HER 2/neu, HER 2, CD340
计算分子量 138 kDa
观测分子量185 kDa
GenBank蛋白编号BC167147
基因名称 HER2/ErbB2
Gene ID (NCBI) 2064
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP04626
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.