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ERC1 Polyclonal antibody
ERC1 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, COLO 320 cells, human testis tissue, Jurkat cells |
| Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22211-1-AP targets ERC1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Immunogen | ERC1 fusion protein Ag17536 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ELKS/RAB6-interacting/CAST family member 1 |
| Synonyms | ELKS, Cast2, KIAA1081, ERC-1, ERC 1 |
| Calculated Molecular Weight | 1116 aa, 128 kDa |
| Observed Molecular Weight | 124-135 kDa |
| GenBank Accession Number | BC132782 |
| Gene Symbol | ERC1 |
| Gene ID (NCBI) | 23085 |
| RRID | AB_11232409 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUD2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERC is an acronym based on previous separate namings of members of this protein family, ELKS, Rab6IP2 and CAST (PMID: 12391317). They are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. ERC1 (ELKS) is an essential regulatory subunit of the IKK complex and likely functions by recruiting IκBα to the complex (PMID: 15218148). Multiple isoforms of ERC1 mRNA are generated by alternative splicing (PMID: 12203787). There are two known splice variants of ERC1 protein that differ at their C termini, ERC1a and ERC1b. ERC1a is ubiquitously expressed, whereas ERC1b is brain-specific (PMID: 12391317; 12923177).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERC1 antibody 22211-1-AP | Download protocol |
| IF protocol for ERC1 antibody 22211-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry. | ||
J Cell Sci Organization and dynamics of the cortical complexes controlling insulin secretion in β-cells. | ||
J Biol Chem MICAL3 flavoprotein monooxygenase forms a complex with centralspindlin and regulates cytokinesis.
| ||
Nat Commun CLASP-mediated competitive binding in protein condensates directs microtubule growth | ||
