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验证数据展示

  • WB analysis of human brain using 21396-1-AP

    WB analysis of human brain using 21396-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21396-1-AP (ERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21396-1-AP

    IF Staining of HeLa using 21396-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERC2 antibody (21396-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human bowen disease using 21396-1-AP

    IHC staining of human bowen disease using 21396-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21396-1-AP (ERC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human bowen disease using 21396-1-AP

    IHC staining of human bowen disease using 21396-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21396-1-AP (ERC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ERC2 Polyclonal antibody

ERC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21396-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA0378, ERC protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IHC detected inhuman bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21396-1-AP targets ERC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERC2 fusion protein Ag16080 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ELKS/RAB6-interacting/CAST family member 2
别名 KIAA0378, ERC protein 2
计算分子量 957 aa, 111 kDa
观测分子量 115-120 kDa
GenBank蛋白编号BC111550
基因名称 ERC2
Gene ID (NCBI) 26059
RRIDAB_10859782
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15083
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ERC2 antibody 21396-1-APDownload protocol
IHC protocol for ERC2 antibody 21396-1-APDownload protocol
IF protocol for ERC2 antibody 21396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols