• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ERCC1 Polyclonal antibody

ERCC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14586-1-AP

Print datasheet

Synonyms

UV20, RAD10, DNA excision repair protein ERCC-1, COFS4

验证数据展示

  • WB analysis using 14586-1-AP

    WB analysis using 14586-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14586-1-AP (ERCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 14586-1-AP

    IHC staining of human testis using 14586-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 14586-1-AP

    IHC staining of human testis using 14586-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of MCF-7 using 14586-1-AP

    IP experiment of MCF-7 using 14586-1-AP

    IP result of anti-ERCC1 (IP:14586-1-AP, 3ug; Detection:14586-1-AP 1:300) with MCF-7 cells lysate 1600ug.

  • IF Staining of MCF-7 using 14586-1-AP

    IF Staining of MCF-7 using 14586-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, human kidney tissue, MCF-7 cells, SKOV-3 cells, T-47D cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14586-1-AP targets ERCC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ERCC1 fusion protein Ag6112 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Synonyms UV20, RAD10, DNA excision repair protein ERCC-1, COFS4
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC052813
Gene Symbol ERCC1
Gene ID (NCBI) 2067
RRIDAB_2100027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07992
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005). The calculated molecular weight of ERCC1 is 33 kDa, but the modified ERCC1 is about38 kDa.

实验方案

Product Specific Protocols
WB protocol for ERCC1 antibody 14586-1-APDownload protocol
IHC protocol for ERCC1 antibody 14586-1-APDownload protocol
IF protocol for ERCC1 antibody 14586-1-APDownload protocol
IP protocol for ERCC1 antibody 14586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Blood Cancer J

Severe ineffective erythropoiesis discriminates prognosis in myelodysplastic syndromes: analysis based on 776 patients from a single centre.

Authors - Huijun Huang
  • KD Validated
View Article
humanWB

Cell Death Dis

PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.

Authors - Wen-Kai Shi
View Article
humanWB

J Cell Mol Med

Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.

Authors - Xingjie Gao
View Article
humanWB

Oncotarget

Functional characterization of a novel transcript of ERCC1 in chemotherapy resistance of ovarian cancer.

Authors - Jia Liu
View Article
humanWB, IHC

Oncol Res

The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1.

Authors - Wei Wei
  • KD Validated
View Article
humanWB

Cancer Cell Int

Mechanistic study of lncRNA UCA1 promoting growth and cisplatin resistance in lung adenocarcinoma.

Authors - Jiali Fu
View Article