验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, A431 cells, COLO 320 cells, HT-1080 cells, mouse colon tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
10580-1-AP targets ERCC3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | ERCC3 fusion protein Ag0876 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) |
| 别名 | EC:5.6.2.4, DNA repair protein complementing XP-B cells, DNA excision repair protein ERCC-3, DNA 3'-5' helicase/translocase XPB, BTF2 p89 |
| 计算分子量 | 89 kDa |
| 观测分子量 | 89 kDa |
| GenBank蛋白编号 | BC008820 |
| 基因名称 | ERCC3 |
| Gene ID (NCBI) | 2071 |
| RRID | AB_2262174 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19447 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Excision repair cross-complementing 3/xeroderma pigmentosum B (ERCC3/XPB) DNA helicase, is an essential ATP-dependent DNA helicase that functions as an integral component of the transcription factor IIH complex. ERCC3 is involved in initiation of basal transcription and global genome repair as well as in transcription-coupled repair (TCR) [PMID:19942596]. At the cellular level, a defect in ERCC3 results in high UV sensitivity, reduced post-UV RNA synthesis recovery and decreased DNA repair due to defective NER [PMID:16947863].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERCC3 antibody 10580-1-AP | Download protocol |
| IHC protocol for ERCC3 antibody 10580-1-AP | Download protocol |
| IP protocol for ERCC3 antibody 10580-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Triptolide Inhibits the AR Signaling Pathway to Suppress the Proliferation of Enzalutamide Resistant Prostate Cancer Cells.
| ||
Biochem Biophys Res Commun MiR-192 inhibits nucleotide excision repair by targeting ERCC3 and ERCC4 in HepG2.2.15 cells. | ||
