验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HeLa cells, PC-3 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24291-1-AP targets ERCC6/CSB in WB, IP, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | ERCC6/CSB fusion protein Ag18009 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | excision repair cross-complementing rodent repair deficiency, complementation group 6 |
| Synonyms | ARMD5, ATP dependent helicase ERCC6, CKN2, Cockayne syndrome protein CSB, COFS, COFS1, CSB, ERCC6, ERCC6/CSB, RAD26 |
| Calculated Molecular Weight | 1493 aa, 168 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | BC127104 |
| Gene Symbol | ERCC6/CSB |
| Gene ID (NCBI) | 2074 |
| RRID | AB_2879476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0DP91 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERCC6/CSB antibody 24291-1-AP | Download protocol |
| IP protocol for ERCC6/CSB antibody 24291-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci A Gene Expression Signature to Predict Nucleotide Excision Repair Defects and Novel Therapeutic Approaches. | ||
Front Mol Biosci Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.
| ||
Life Sci Alliance Elevated glucose increases genomic instability by inhibiting nucleotide excision repair. | ||
Nucleic Acids Res Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons |
