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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 26468-1-AP

    WB analysis using 26468-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26468-1-AP (ERF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 26468-1-AP

    IP experiment of A431 using 26468-1-AP

    IP result of anti-ERF (IP:26468-1-AP, 4ug; Detection:26468-1-AP 1:2000) with A431 cells lysate 1040 ug.

ERF Polyclonal antibody

ERF Polyclonal Antibody for WB, IP, ELISA
Cat No. 26468-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

PE2, PE 2, Ets2 repressor factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A549 cells
Positive IP detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26468-1-AP targets ERF in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen ERF fusion protein Ag24189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ets2 repressor factor
Synonyms PE2, PE 2, Ets2 repressor factor
Calculated Molecular Weight 548 aa, 59 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC022231
Gene Symbol ERF
Gene ID (NCBI) 2077
RRIDAB_3669537
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50548
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERF (Ets2 repressor factor) is also named as PE-2. Erf is a gene for a ubiquitously expressed Ets DNA-binding domain-containing transcriptional repressor regulated by Erk-dependent phosphorylation (PMID: 28694332, PMID: 35258130). Erf is involved in T cell maturation, acting as a positive regulator during CD4 and eventually CD8 lineage commitment, while negatively regulates the production of γδ T cells (PMID: 35258130). Erf is required for both primitive and erythromyeloid progenitor waves of hematopoietic stem cell (HSC)-independent hematopoiesis as well as for the normal function of HSCs (PMID: 28694332). ETS2 repressor factor (ERF) insufficiency causes craniosynostosis (CRS4) in humans and mice. ERF is an ETS domain transcriptional repressor regulated by Erk1/2 phosphorylation via nucleo-cytoplasmic shuttling (PMID: 37175668).

实验方案

Product Specific Protocols
WB protocol for ERF antibody 26468-1-APDownload protocol
IP protocol for ERF antibody 26468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols