验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, A549 cells |
Positive IP detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26468-1-AP targets ERF in WB, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IP, ELISA Application Description |
经测试反应性 | human |
免疫原 | ERF fusion protein Ag24189 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Ets2 repressor factor |
别名 | PE2, PE 2, Ets2 repressor factor |
计算分子量 | 548 aa, 59 kDa |
观测分子量 | 90 kDa |
GenBank蛋白编号 | BC022231 |
基因名称 | ERF |
Gene ID (NCBI) | 2077 |
RRID | AB_3669537 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P50548 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERF (Ets2 repressor factor) is also named as PE-2. Erf is a gene for a ubiquitously expressed Ets DNA-binding domain-containing transcriptional repressor regulated by Erk-dependent phosphorylation (PMID: 28694332, PMID: 35258130). Erf is involved in T cell maturation, acting as a positive regulator during CD4 and eventually CD8 lineage commitment, while negatively regulates the production of γδ T cells (PMID: 35258130). Erf is required for both primitive and erythromyeloid progenitor waves of hematopoietic stem cell (HSC)-independent hematopoiesis as well as for the normal function of HSCs (PMID: 28694332). ETS2 repressor factor (ERF) insufficiency causes craniosynostosis (CRS4) in humans and mice. ERF is an ETS domain transcriptional repressor regulated by Erk1/2 phosphorylation via nucleo-cytoplasmic shuttling (PMID: 37175668).
实验方案
Product Specific Protocols | |
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WB protocol for ERF antibody 26468-1-AP | Download protocol |
IP protocol for ERF antibody 26468-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |