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验证数据展示

  • IP experiment of HepG2 using 11927-1-AP

    IP experiment of HepG2 using 11927-1-AP

    IP result of anti-ERGIC2 (IP:11927-1-AP, 4ug; Detection:11927-1-AP 1:1000) with HepG2 cells lysate 2900ug.

  • IHC staining of human prostate cancer using 11927-1-AP

    IHC staining of human prostate cancer using 11927-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 11927-1-AP

    IF Staining of HepG2 using 11927-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 11927-1-AP

    WB analysis using 11927-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11927-1-AP (ERGIC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 11927-1-AP

    WB analysis of HeLa using 11927-1-AP

    WB result of ERGIC2 antibody (11927-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC2 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

ERGIC2 Polyclonal antibody

ERGIC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11927-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PTX1, ERV41, ERGIC and golgi 2, Endoplasmic reticulum-Golgi intermediate compartment protein 2, CDA14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11927-1-AP targets ERGIC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 ERGIC2 fusion protein Ag2603 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ERGIC and golgi 2
别名 PTX1, ERV41, ERGIC and golgi 2, Endoplasmic reticulum-Golgi intermediate compartment protein 2, CDA14
计算分子量 377 aa, 43 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC000887
基因名称 ERGIC2
Gene ID (NCBI) 51290
RRIDAB_2098440
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RQ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERGIC2, also named as Endoplasmic reticulum-Golgi intermediate compartment protein 2 or PTX1, is a 377 amino acid protein, which belongs to the ERGIC family. ERGIC2 is ubiquitously expressed in many tissues. ERGIC2 localizes in the endoplasmic reticulum-Golgi intermediate compartment membrane and may play a role in transport between endoplasmic reticulum and Golgi. ERGIC2 is involved in protein transportation, and participated in the elongation factor 1 alpha regulated mechanisms.

实验方案

Product Specific Protocols
WB protocol for ERGIC2 antibody 11927-1-APDownload protocol
IHC protocol for ERGIC2 antibody 11927-1-APDownload protocol
IF protocol for ERGIC2 antibody 11927-1-APDownload protocol
IP protocol for ERGIC2 antibody 11927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

DNA Cell Biol

Characterization of a Variant of ERGIC2 Transcript.

Authors - Kwok Simon C M SC
View Article