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验证数据展示

  • WB analysis using 16029-1-AP

    WB analysis using 16029-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16029-1-AP (ERGIC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16029-1-AP

    IHC staining of mouse brain using 16029-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16029-1-AP (ERGIC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16029-1-AP

    IHC staining of mouse brain using 16029-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16029-1-AP (ERGIC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 16029-1-AP

    WB analysis of HepG2 using 16029-1-AP

    WB result of ERGIC3 antibody (16029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC3 transfected HepG2 cells.

  • IF Staining of HepG2 using 16029-1-AP

    IF Staining of HepG2 using 16029-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ERGIC3 antibody (16029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

ERGIC3 Polyclonal antibody

ERGIC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16029-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

NY BR 84, ERV46, Endoplasmic reticulum-Golgi intermediate compartment protein 3, CGI-54, CGI 54

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, MCF-7 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16029-1-AP targets ERGIC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ERGIC3 fusion protein Ag8893 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ERGIC and golgi 3
别名 NY BR 84, ERV46, Endoplasmic reticulum-Golgi intermediate compartment protein 3, CGI-54, CGI 54
计算分子量 383 aa, 43 kDa
观测分子量 50 kDa
GenBank蛋白编号BC009765
基因名称 ERGIC3
Gene ID (NCBI) 51614
RRIDAB_2098446
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y282
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERGIC3 (Endoplasmic reticulum-Golgi intermediate compartment protein 3 ) is located in the cis face of the Golgi apparatus and vesicular tubular structures between the transitional endoplasmic reticulum (ER) and cis-Golgi. ERGIC3 significantly affects cell growth and causes ER stress-induced cell death, and is involved in the invasion and metastasis in hepatocellular carcinomas (HCC). (PMID: 26177443, PMID: 31142615)

实验方案

Product Specific Protocols
WB protocol for ERGIC3 antibody 16029-1-APDownload protocol
IHC protocol for ERGIC3 antibody 16029-1-APDownload protocol
IF protocol for ERGIC3 antibody 16029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Identification of TRAMs as Sphingolipid-Binding Proteins Using a Photoactivatable and Clickable Short Chain Ceramide Analog.

Authors - Yaqin Deng
View Article