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验证数据展示

  • WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

    WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ERK1/2 antibody included
  • IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ERK1/2 antibody included
  • IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

    IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ERK1/2 antibody (51068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

ERK1/2 Polyclonal antibody, PBS Only

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 51068-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ERK1/2 Ready-To-Use IHC Kit. ERK1/2 primary antibody included.

产品信息

51068-1-PBS targets ERK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 1
别名
计算分子量 360 aa, 41 kDa
观测分子量 38-44 kDa
GenBank蛋白编号BC017832
基因名称 ERK2
Gene ID (NCBI) 5594
RRIDAB_2250380
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28482
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.