• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ERK1/2 Polyclonal antibody, PBS Only

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51068-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

    WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ERK1/2 antibody included
  • IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ERK1/2 antibody included
  • IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

    IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ERK1/2 antibody (51068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ERK1/2 Ready-To-Use IHC Kit. ERK1/2 primary antibody included.

产品信息

51068-1-PBS targets ERK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 1
Synonyms
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight 38-44 kDa
GenBank Accession NumberBC017832
Gene Symbol ERK2
Gene ID (NCBI) 5594
RRIDAB_2250380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28482
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.