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ERMAP Polyclonal antibody

ERMAP Polyclonal Antibody for IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21375-1-AP

Print datasheet

Synonyms

ERMAP, hERMAP, PRO2801, Radin blood group antigen, RD, SC, Scianna blood group antigen

验证数据展示

  • IP experiment of MCF-7 using 21375-1-AP

    IP experiment of MCF-7 using 21375-1-AP

    IP result of anti-ERMAP (IP:21375-1-AP, 4ug; Detection:21375-1-AP 1:300) with MCF-7 cells lysate 1600ug.

  • IF Staining of MCF-7 using 21375-1-AP

    IF Staining of MCF-7 using 21375-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 21375-1-AP (ERMAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IP detected inMCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 21375-1-AP is ERMAP Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen ERMAP fusion protein Ag15899 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name erythroblast membrane-associated protein (Scianna blood group)
Synonyms ERMAP, hERMAP, PRO2801, Radin blood group antigen, RD, SC, Scianna blood group antigen
Calculated Molecular Weight 475 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC099703
Gene Symbol ERMAP
Gene ID (NCBI) 114625
RRIDAB_2878849
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PL5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ERMAP antibody 21375-1-APDownload protocol
IP protocol for ERMAP antibody 21375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Immunol

The ligation between ERMAP, galectin-9 and dectin-2 promotes Kupffer cell phagocytosis and antitumor immunity

Authors - Jie Li
View Article