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验证数据展示

  • WB analysis of JAR using 67289-1-Ig

    WB analysis of JAR using 67289-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67289-1-Ig (ERN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67289-1-Ig

    IHC staining of human liver cancer using 67289-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67289-1-Ig (ERN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67289-1-Ig

    IHC staining of human liver cancer using 67289-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67289-1-Ig (ERN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ERN2 Monoclonal antibody

ERN2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67289-1-Ig

产品说明书

CloneNo. 2G6A10

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IHC, ELISA

别名

ERN2, hIRE2p, Inositol requiring protein 2, Ire1 beta, IRE1b, IRE2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJAR cells, A549 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67289-1-Ig targets ERN2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 ERN2 fusion protein Ag29452 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 endoplasmic reticulum to nucleus signaling 2
别名 ERN2, hIRE2p, Inositol requiring protein 2, Ire1 beta, IRE1b, IRE2
观测分子量 102 kDa
GenBank蛋白编号BC157113
基因名称 ERN2
Gene ID (NCBI) 10595
RRIDAB_2882555
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ76MJ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ERN2 antibody 67289-1-IgDownload protocol
IHC protocol for ERN2 antibody 67289-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols