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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of mouse ovary using 12007-1-AP (same clone as 12007-1-PBS)

    WB analysis of mouse ovary using 12007-1-AP (same clone as 12007-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12007-1-AP (ERO1L antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12007-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 12007-1-AP (same clone as 12007-1-PBS)

    IHC staining of human pancreas cancer using 12007-1-AP (same clone as 12007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12007-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 12007-1-AP (same clone as 12007-1-PBS)

    IHC staining of human pancreas cancer using 12007-1-AP (same clone as 12007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12007-1-PBS in a different storage buffer formulation.

ERO1L Polyclonal antibody, PBS Only

ERO1L Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12007-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12007-1-PBS targets ERO1L in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERO1L fusion protein Ag2620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ERO1-like (S. cerevisiae)
别名
计算分子量 468 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC008674
基因名称 ERO1L
Gene ID (NCBI) 30001
RRIDAB_10666441
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HE7
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. This antibody has no cross reaction to ERO1.