验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human placenta tissue |
| Positive IHC detected in | human pancreas cancer tissue, human oesophagus tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
产品信息
11261-2-AP targets ERO1LB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ERO1LB fusion protein Ag1776 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ERO1-like beta (S. cerevisiae) |
| 别名 | ERO1-L-beta, Ero1beta, ERO1B, Endoplasmic reticulum oxidoreductin-1-like protein B, Endoplasmic reticulum oxidoreductase beta |
| 计算分子量 | 54 kDa |
| 观测分子量 | 40~50 kDa |
| GenBank蛋白编号 | BC032823 |
| 基因名称 | ERO1LB |
| Gene ID (NCBI) | 56605 |
| RRID | AB_2101503 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86YB8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Mammals express two related Ero proteins, Ero1L alpha and Ero1L beta. The two homologous proteins can form homodimers and mixed heterodimers. ERO1-Lα and -β are highly expressed in organs with a high demand for secretion, consistent with their roles in oxidative folding of ER proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERO1LB antibody 11261-2-AP | Download protocol |
| IHC protocol for ERO1LB antibody 11261-2-AP | Download protocol |
| IF protocol for ERO1LB antibody 11261-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Curcumin Facilitates a Transitory Cellular Stress Response in Trembler-J Mice. | ||
J Biol Chem Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells. | ||
Sci Rep Hypoxia-inducible ERO1α promotes cancer progression through modulation of integrin-β1 modification and signalling in HCT116 colorectal cancer cells.
| ||
J Toxicol Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease. | ||
ACS Bio Med Chem Au Identification of Natural Product Sulfuretin Derivatives as Inhibitors for the Endoplasmic Reticulum Redox Protein ERO1α
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