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验证数据展示

  • WB analysis of human brain using 18312-1-AP

    WB analysis of human brain using 18312-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 18312-1-AP

    IHC staining of human pancreas using 18312-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 18312-1-AP

    IHC staining of human pancreas using 18312-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 40x lens).

ERO1LB Polyclonal antibody

ERO1LB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18312-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ERO1-L-beta, Ero1beta, ERO1B, Endoplasmic reticulum oxidoreductin-1-like protein B, Endoplasmic reticulum oxidoreductase beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18312-1-AP targets ERO1LB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ERO1-like beta (S. cerevisiae)
别名 ERO1-L-beta, Ero1beta, ERO1B, Endoplasmic reticulum oxidoreductin-1-like protein B, Endoplasmic reticulum oxidoreductase beta
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC032823
基因名称 ERO1LB
Gene ID (NCBI) 56605
RRIDAB_2101502
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86YB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERO1LB is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. It is required for the folding of cell, thereby being a source of oxidative stress. This antibody has no cross reaction to ERO1L.

实验方案

Product Specific Protocols
WB protocol for ERO1LB antibody 18312-1-APDownload protocol
IHC protocol for ERO1LB antibody 18312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols