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验证数据展示

  • WB analysis of human brain using 18312-1-AP

    WB analysis of human brain using 18312-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 18312-1-AP

    IHC staining of human pancreas using 18312-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 18312-1-AP

    IHC staining of human pancreas using 18312-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 40x lens).

ERO1LB Polyclonal antibody

ERO1LB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18312-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ERO1-L-beta, Ero1beta, ERO1B, Endoplasmic reticulum oxidoreductin-1-like protein B, Endoplasmic reticulum oxidoreductase beta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18312-1-AP targets ERO1LB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ERO1-like beta (S. cerevisiae)
Synonyms ERO1-L-beta, Ero1beta, ERO1B, Endoplasmic reticulum oxidoreductin-1-like protein B, Endoplasmic reticulum oxidoreductase beta
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC032823
Gene Symbol ERO1LB
Gene ID (NCBI) 56605
RRIDAB_2101502
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERO1LB is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. It is required for the folding of cell, thereby being a source of oxidative stress. This antibody has no cross reaction to ERO1L.

实验方案

Product Specific Protocols
WB protocol for ERO1LB antibody 18312-1-APDownload protocol
IHC protocol for ERO1LB antibody 18312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols